Effect of siRNA depletion of Nrf2 on proliferation of FAMPAC, MiaPaca-2 and Suit-2 pancreatic cancer cells. Cells were transfected with 10 nM Nrf2-targeting siRNA, or Stealth RNAi control, for up to 120 h. A, Cell survival was measured at 120 h using the MTS test. Data is shown as cell viability versus Stealth RNAi control. B, Cell numbers were quantified at the indicated time points using Trypan blue staining. Data are the means ± S.D. of four discrete experiments. ** = P < 0.01,*** = P < 0.001.