Effect of siRNA depletion of Nrf2 on sensitivity of Suit-2 and FAMPAC pancreatic cancer cells to chemo- and radiotherapies. Cells were transfected with 10 nM Nrf2-targeting siRNA, or Stealth RNAi control, for 48 h followed by exposure to gemcitabine (A), 5 flurouracil (5-FU) (B), cisplatin (C) or gamma radiation (D) for 72 h, at the indicated concentrations. Cell viability was measured using the MTS assay. Graphs represent data as a surviving fraction versus non-drug treated Stealth RNAi control transfected cells. IC50 values are expressed as μM. Data are the means ± S.D. of four discrete experiments. * = P < 0.05,** = P < 0.01,*** = P < 0.001.