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Figure 8 | Molecular Cancer

Figure 8

From: High susceptibility of metastatic cells derived from human prostate and colon cancer cells to TRAIL and sensitization of TRAIL-insensitive primary cells to TRAIL by 4,5-dimethoxy-2-nitrobenzaldehyde

Figure 8

Suppression of DNA-PKcs leads to activation of caspase and Bax through the inactivation of the DNA-PKcs/Akt signaling pathway and enhances responsiveness to TRAIL. (A) PC3 (left) and KM12 cells (right) were transfected with siRNA against DNA-PKcs or scrambled siRNA as a control. After 48 h, the transfected PC3 and KM12 cells treated with the indicated doses of TRAIL for 6 and 8 h, respectively, and were subjected to Western blot analysis to monitor the levels of DNA-PKcs, pAkt, tAkt, caspases (caspase-8, -9, and -3) and Bax. The levels of PARP and its cleavage fragment (CF) in the transfectants were also determined. Actin was used as a loading control. (B) PC3 (left) and KM12 cells (right) were transfected with siRNA against DNA-PKcs or scrambled siRNA as a control. After 48 h, the transfected cells were treated with the indicated doses of TRAIL for 6 and 8 h, respectively. Thereafter, the percentage of apoptotic cells was determined using Annexin V staining and flow cytometry (right). Each value represents the mean ± SE of triplicate determinants. *p < 0.05, **p < 0.01.

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