Role of nutlin-induced MDM2 in AR degradation. A) Nutlin-induced MDM2 facilitates the degradation of MDMX but not AR. LNCaP cells were grown in CSS in the presence or absence of 5 μM Nutlin-3a (24 h) and/or MG132 (8 h) and proteins were analyzed by Western blotting. B) LNCaP cell clone stably expressing high levels of MDM2 show a reduction in AR levels. Cell lysates from LNCaP cells transfected with a vector (V) and a clone stably expressing a full length MDM2 protein from an exogenous CMV-driven expression construct (M) were analyzed by Western blotting.