Figure 7
From: Mutation of NIMA-related kinase 1 (NEK1) leads to chromosome instability
Anueploidy in lymphoid tumors from older NEK1 +/- mice. Cells from the spleens of a 9 month-old wild type mouse and a NEK1 +/- mice littermate, 3 month-old NEK1 +/- mice, and three lymphoid tumors were fixed and stained with propidium iodide, and then analyzed by FACS for DNA content. Percentages of 2n cells from the spleen from old NEK1 +/- mice and from a lymphoma were decreased compared to cells from the spleens of wild type and young NEK1 +/- mice. Cells with DNA content greater than 4n were increased in the spleen cells from old NEK1 +/- mice and in the lymphoid tumors. In the lymphoid tumor shown, note also the relatively large number of cells, characterized by high valleys in the FACS plots, with non-integer DNA content. See also additional file 1-Fig. S1.