Figure 5
From: Lipid phosphate phosphatase-3 regulates tumor growth via β-catenin and Cyclin-D1 signaling
LPP3 regulates glioblastoma tumor growth. (A) Stable clones of U87 and U118 were selected and efficiency of knockdown was determined by western blotting with the indicated antibodies. LPP3-knockdown decreased total β-catenin, CYCLIN-D1, and CD133 proteins, whereas it had no effect on LPP2 or GAPDH. (B) Conditioned media were collected and subjected to ELISA for VEGF, IL-8, and TIMP-2. LPP3-knockdown in U87 and U118 cells reduced the concentration of VEGF and IL-8, whereas TIMP-2 was unaffected. (C) In the xenograft assay, mice receiving cells that expressed control shRNA showed increased tumor growth (n = 12, ¥ P < 0.05) after 14 and 28 days. LPP3-knockdown reduced U87 and U118 tumor growth (n = 12, ¶ P < 0.05). (D) Representative images from the xenograft assay. Dotted circles indicate the location of tumor implants.