Figure 7
From: IκB kinases increase Myc protein stability and enhance progression of breast cancer cells
IKK activity is necessary for doxorubicin to increase Myc, cyclin D1 and twist protein levels in MCF7 cells. The cells (A) MCF/IKKα-WT, (B) MCF/IKKα-KD, (C) MCF/IKKβ-WT, and (D) MCF/IKKβ-KD were treated with 200 nM doxorubicin for 3hours, and then were released with complete medium for further 24 hours. The cytoplasmic and nuclear extracts were subjected to Western blot analysis.