Figure 5
From: Natriuretic Peptide Receptor A as a Novel Target for Prostate Cancer
iNPRA treatment reduces tumor burden by inhibiting MIF expression. (A-B) Effects of iNPRA in TRAMP-C1 inoculated xenografts in immunocompetent mice. Four groups of C57BL/6 mice (n = 7 per group) were injected s.c. in the right flank with 5 × 106 TRAMP-C1 cells. Two weeks later, tumor-inoculated mice were treated with CNPs encapsulated with pVAX, pNP73-102, pVD or a combination of pNP73-102 and pVD i.p. twice a week until euthanized. The tumor size (A) was measured at the indicated days, and the weight was recorded after tumor resection (B). (C) NPRA expression correlates with MIF expression in tumor lysates. Tumor lysates from B were analysed for NPRA and MIF by western blotting. pVAX (lanes 1-3) and pNP73-102 (lanes 4-6). β-actin was used as a loading control.