SiDaam1 reverses the anti-tumor effects of antagomir-335 in C6 cells. (A) SiDaam1 obviously abrogated upregulation of DAAM1 induced by antagomir-335 transfection. (B-E) siDaam1 counteracted the anti-tumor effects of antagomir-335. SiDaam1 significantly abrogated antagomir-335-induced growth arrest (B), repression of colony formation(C), invasion inhibition (D) and caspase3/7 activity (E). C6 cells were transfected with 50 nM siDaam1 and/or 100 nM antagomir-335 for the indicated times. Results represent the means ± SD for three repeats. (***, p < 0.001). Original magnification in (D), 200 ×.