Figure 7
From: Targeting oncogenic miR-335 inhibits growth and invasion of malignant astrocytoma cells
MiR-335 inhibition suppresses tumor growth in vivo. (A-C) Pretreated effect of antagomir-335 on tumor formation in nude mouse xenograft model. (A)Antagomir-NC-transfected and antagomir-335-transfected C6 cells were s.c. injected into the left (up-panel) and right (down-panel) flanks of nude mice, respectively (n = 8). (B) Tumor volume was monitored during the time course of 19 days. (C) Tumor weight was detected at the end of the study. (D-H) Therapeutic effect of antagomir-335 on tumor growth in nude mouse xenograft model. (D) Tumors generated by C6 cells injection in nude mice were intratumorally injected with antagomir-NC (up-panel) and antagomir-335 (down-panel) for 2 weeks, respectively(n = 5). (E) Tumor volume was monitored during the time course of 4 weeks. (F) Tumor weight was detected at the end of the study. (G) DAAM1 expression was detected by immunohistochemistry. (H) Apoptotic cells were detected by TUNEL assay. Data represent the means ± SD. Statistical differences compared with the controls are given as **, P < 0.01; ***, P < 0.001. Original magnification in (G up-panel), 320 ×; Original magnification in (H), 400 ×.