Figure 5

CXCR7 expression by HUVEC is dispensable for CCX771 blockade of cell TEM. A: HUVEC were transfected with CXCR7-targeted RNAi (black open histogram) or scramble-RNAi (red open histogram) oligonucleotides and analyzed by flow cytometry with the CXCR7-specific mAb 11G8. The filled histogram shows isotype control mAb staining. B: NC-37 cell TEM across CXCR7-targeted RNAi (left graph) or scramble-RNAi (right graph) HUVEC to 10 nM CXCL12 was performed in the presence of CCX771 or CCX704 (red bars). Controls (black bars) include TEM in the absence of CXCL12 or the presence of CXCL12 but the absence of compounds. C: NC-37 cell TEM across CXCR7-targeted RNAi (left graph) or scramble-RNAi (right graph) HUVEC to 300 nM CXCL13 in the presence of 10 nM CXCL12 in both wells was performed in the additional presence of CCX771 or CCX704 (red bars). Controls (black bars) include TEM in the absence of chemokines, presence of CXCL13 only, presence of CXCL12 only, and presence of CXCL13 and CXCL12 but the absence of compounds. * p-value < 0.05 by Student's t-test comparing the indicated treatment vs. matched control. For B and C, the mean ± SEM of quadruplicate wells per treatment is shown. Representative of 2 independent experiments.