Figure 6

Hyperactivation of β-catenin/TCF-dependent transcription induced by Wnt-3a and HFz1 in the context of E6 variants. (A) HEK293T cells were co-transfected with TCF/LUC reporter (1 μg), β-Gal (0.1 μg), Hfz1 (0.3 μg), Wnt-3a (0.1 μg) and E6 variants as indicated. Each E6/variant was transfected at 2 amounts, 0.25, 0.5 μg plasmid DNA. (B) C33A cells were transfected as in indicated in A except that 0.5 Renilla luciferase DNA was transfected instead of β-Gal. Luciferase activities were measured 48 h following transfection. The histograms show fold activation relative to the control cells transfected with the vector DNA (pJS55). The average values of fold activation ± SD of the indicated plasmids are shown. Data are from at least 3 independent experiments with HEK293T and two experiments with C33A cells. Significance was tested by a one-way ANOVA test. A P value of < 0.05 was considered significant.