Differential expression of Cyr61 mRNA and protein in Normal pancreas and pancreatic ductal adenocarcinoma tissue samples. A. The photomicrographs (upper panel and lower panel) represents the distribution pattern of Cyr61 mRNA (blue color) by in situ hybridization using digoxigenin (DIG)-labeled PCR generated Cyr61 specific non-radioactive probe and Cyr61 protein (brown color) by immunohistochemistry in a tissue array containing adjacent normal pancreas, and different grades of ductal adenocarcinoma (Gr-I, Gr-II and Gr-III) respectively. Scale bars: 100μm. B. Distribution profile and labeling intensity of Cyr61 protein in PDAC tissue samples. Gr-I, Grade 1, Gr-II, Grade II and Gr-III, Grade III. (-), No stain, (+), light brown, (++), moderately dark brown, and (+++), dark brown. C. H & E staining and immunohistochemical identification of Cyr61 in precursor lesions of pancreatic cancers. Scale bars: 50μm.