Figure 1

DNA promoter hypermethylation status of 13 candidate genes in colon cancer cell lines. Colon cancer cell lines were used as in vitro models to explore the DNA promoter methylation frequency of 13 candidate biomarkers. Only candidates with methylation frequencies equal to or higher than 80% (CNRIP1, INA, BEX1, FBN1, SNCA, and C3orf14) were subjected to methylation analysis in tissue samples.