E2 box-binding repressor expression upon TGF-β stimulation. PANC-1 and MDA-MB-231 cells were treated with recombinant human TGF-β1 for four days. mRNA expression of CXADR (CAR) (A), CDH1 (E-cadherin) (B), ZEB2 (SIP1) (C), ZEB1 (D), SNAI1 (Snail) (E), and SNAI2 (Slug) (F) was measured by TaqMan real-time PCR. Expression data were normalized to H3F3A (H3 histone, family 3A) mRNA levels (arbitrary units). A-F. Error bars represent standard deviations (biological triplicates). p < 0.001 (***), p < 0.01 (**), p < 0.05 (*) (Student's t-test: 1-tailed, equal variance). Absence of * indicates p ≥ 0.05, or decrease of E2 box-binding repressor expression upon TGF-β stimulation.