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Figure 4 | Molecular Cancer

Figure 4

From: A nucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression

Figure 4

TAS106 suppressed the expression of BRCA2 and Rad51 at protein and mRNA levels in A549 cells. (A) Effects of TAS106 on the expression levels of DNA repair-related proteins. Cells were treated with TAS106 (1 μM) for 12 or 24 h. After incubation, cell extracts were analyzed by Western blotting using specific antibodies. Actin was used as a loading control. (Left) Representative blots of three separate experiments are shown. (Right) Bands corresponding to each protein were quantified, and intensities of each protein were normalized to the intensity of actin. Data are expressed as mean ± SE of three experiments. (B) Effects of TAS106 on the mRNA expression levels of DNA repair-related proteins. Cells were treated with TAS106 (1 μM) for 12 or 24 h. After incubation, total RNA was isolated and the mRNA expression was analyzed by RT-PCR. Actin was used as an internal control. (Left) A representative image of three separate experiments is shown. (Right) Bands corresponding to each mRNA were quantified, and the intensity of each mRNA was normalized to the intensity of actin. Data are expressed as mean ± SE of three experiments.

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Molecular Cancer

ISSN: 1476-4598