Figure 6

Src and Src^P416 recruitment to MUC1 after manipulation of dimerization. Co-immunoprecipitation with anti-MUC1-CD was performed on 293T MUC1-CFP-Fv cells (A, B) and MUC1-CFP cells (C, D) after treatment with increasing concentrations of AP20187^D (A, C) or AP21998^M (B, D). Immunoprecipitates were probed for Src^P416, Src, and anti-MUC1-CD (as a loading control) with stripping of blots between each probe. Whole cell lysates (WCL), PG (Protein G) + Antibody and Antibody only lanes are included as controls. Densitometric analysis of Src and Src^P416 compared to MUC1-CD is presented in Additional File 1.