Figure 4From: Regulation of the stem cell marker CD133 is independent of promoter hypermethylation in human epithelial differentiation and cancerMethylation of the CD133 promoter in prostate primary tissues and xenografts. Methylation analysis of the CD133 promoter in primary prostate cancer xenografts generated in RAG2-/- gamma C-/- mice (A), in PC3 xenografts (D) and in prostate tissues (DNA extracted from glass slides of pooled snap frozen tissue sections) (E) and by pyrosequencing (PYRO 1 assay; bars = single CpG sites; n = 3 technical replicas; ± SD; black line = average of 10 CpG sites; * = p < 0.05; ** = p < 0.01; *** = p < 0.001 in an unpaired t-test). (B) Representative image of CD133 expression measured by FACs in disaggregated primary prostate cancer xenografts. X axes: FITC channel fluorescence (not stained); Y axes: APC channel fluorescence (CD133-APC); Percent of CD133+ cells is indicated. (D) qRT-PCR analysis (relative to RPLP0, calibrator = PC3; N.D. = expression undetectable after 40 cycles) of CD133 expression in PC3 xenografts compared to PC3 (RC-165N/hTERT was used as a positive control).Back to article page