Association between BMI-1 expression and invasive potential and EMT features of EC cell lines. A. BMI-1 protein levels in the panel of EC cell lines were determined by Western blot analysis. B. Invasive ability of EC cells was evaluated by Matrigel invasion assays. Data represent the mean ± SE of three independent experiments. C. Highly invasive HEC-50B-HI cells exhibiting fibroblastic morphology and the parental HEC-50B cells showing epithelial-like appearance were fixed with neutral-buffered formalin, stained in Giemsa, and images were taken (magnification × 100). D. Western blot analysis of epithelial marker E-cadherin and mesenchymal marker Vimentin in HEC-50B and HEC-50B-HI cells.