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Figure 3 | Molecular Cancer

Figure 3

From: MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1

Figure 3

MiR-194 directly targets BMI-1, and reverses invasive, EMT phenotype in EC cells. A. Sequence of miR-194 binding site in the BMI-1 3'UTR predicted with TargetScan. B. MiR-194 expression levels in HEC-50B and HEC-50B-HI cells were determined by Real-time RT-PCR. Data represent the mean ± SE of three independent experiments. *P < 0.01. C. Western blotting analysis of BMI-1, E-cadherin and Vimentin in un-transfected EC cells and EC cells transfected with miR-194 or control miRNA. D. HEC-50B-HI and HHUA cells were transfected with WT or MT BMI-1 3'UTR luciferase vectors, along with miR-194, miR-128 or control miRNA. Luciferase activity was measured at 24 h after transfection. Data represent the mean ± SE of three independent experiments. *P < 0.01. E. Invasive potential of un-transfected and EC cells transfected with miR-194 or control miRNA was evaluated by Matrigel invasion assays. Data represent the mean ± SE of three independent experiments. *P < 0.01.

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Molecular Cancer

ISSN: 1476-4598