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Figure 1 | Molecular Cancer

Figure 1

From: Systems-wide RNAi analysis of CASP8AP2/FLASH shows transcriptional deregulation of the replication-dependent histone genes and extensive effects on the transcriptome of colorectal cancer cells

Figure 1

RNAi identifies CASP8AP2 / FLASH and NUP62 as essential for the viability of SW480 cells. (A) The relative viability of SW480 cells following transfection of three different siRNAs targeting each of the genes shown (see Methods for the identity of each siRNA). Data is shown as the mean ± SD of three independent transfections for each siRNA targeting the six genes of interest, normalized to the average viability of SW480 cells transfected with the negative control siRNA (siNeg; eight independent transfections). The normalized data for the positive control siRNA, siPLK1, (six independent transfections) is also shown. The statistical comparison (t-test of unequal variance) of siNeg transfected cells to cells transfected with the siRNAs corresponding to the stated genes is indicated by *** = p ≤ 0.001, ** = p ≤0.01, and * = p ≤0.05. (B) The effect of silencing (i) CASP8AP2/FLASH and (ii) NUP62, over 144 hours, on cell viability and their respective mRNA levels in SW480. The data shown represents the mean and standard deviation for three transfections per condition per time point normalized to the data from cells transfected with the negative control siRNA, siNeg. (C) The effect of silencing CASP8AP2/FLASH and NUP62 on their respective mRNA levels (i and iii) and on cell viability (ii and iv) in three SW480, SW837 and SW48 colorectal cancer cell lines. The data shown represents the mean and standard deviation for three transfections per condition per time point normalized to the data from cells transfected with the negative control siRNA, siNeg.

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Molecular Cancer

ISSN: 1476-4598