Figure 6
The loss of CASP8AP2 / FLASH function induces consistent effects on the transcriptome of CRC cells. (A) Heat map representation of the median fold change for those transcripts showing a significant fold change (> ± 0.6 Log2 fold change, p < 0.05) at 72 hours post-transfection for all three siRNAs corresponding to CASP8AP2/FLASH when compared to the expression profiles obtained for siNeg transfected SW480 cells generated at the same time points. Data at earlier time points reflects the fold changes seen for all of these transcripts, though these changes in expression may have not reached significance. (B) The enrichment of functional ontologies for upregulated genes with the CASP8AP2/FLASH RNAi signatures obtained 48 and 72 hours post siRNA transfection. No statistically significant enrichment was seen for genes showing a downregulation in expression within the CASP8AP2/FLASH RNAi signature. (C) Gene set enrichment analysis of the human histone genes at 10, 24, 48 and 72 hours. (D and E) Gene set enrichment analysis of the targets of transcription factors associated with CRC. (D) Shows the overall statistical analysis for the enrichment of the targets within the CASP8AP2/FLASH RNAi signature for six transcription factors, (E)(i) shows the enrichment plots for the targets of NFκB over time, (ii) shows the enrichment plots for the upregulated targets of the MYC transcription factor over time, and (iii) shows the enrichment plots for the downregulated targets of the MYC transcription factor over time.