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Figure 2 | Molecular Cancer

Figure 2

From: Klotho-beta overexpression as a novel target for suppressing proliferation and fibroblast growth factor receptor-4 signaling in hepatocellular carcinoma

Figure 2

Silencing of KLB with siRNA suppresses cell viability and proliferation. Huh7 cells were transfected with 100 nM scrambled control or KLB-targeting siRNA. (A) After 48 h, qRT-PCR was performed to confirm KLB knockdown after normalizing to GAPDH. Error bars represent SD converted to fold changes. (B) At 48 h post transfection, Huh7 cells were re-seeded in a 96-well plate. After additional 24 h incubation, cell viability was measured using CellTiter-Glo and evaluated relative to cells transfected with scrambled siRNA. (C) At the indicated time points after transfection, Huh7 cells were harvested for counting. (D) Immunoblotting with PCNA antibody was performed 72 h post siRNA transfection using tubulin as loading control. For A-C, means of three independent experiments are indicated with SD as error bars. (***, P < 0.001; *, P < 0.05 using Student's t-test).

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Molecular Cancer

ISSN: 1476-4598