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Figure 5 | Molecular Cancer

Figure 5

From: Klotho-beta overexpression as a novel target for suppressing proliferation and fibroblast growth factor receptor-4 signaling in hepatocellular carcinoma

Figure 5

CD133 and CD44 expression are upregulated in PD173074-resistant cells. Resistant Huh7 cells were generated by culturing in sub-lethal concentrations of PD173074 (0.1 μM - 1.0 μM). After eight weeks, (A) CD133 and (B) CD44 mRNA expression were analyzed by qRT-PCR. Results are indicated with SD converted to fold changes as error bars. The differences were statistically significant (P < 0.001 by one-way ANOVA, P < 0.05 by Tukey's multiple comparison test). (C) CD133 protein expression was determined by immunoblotting and normalized to β-actin. (D) Long term effect of PD173074 treatment on FGFR4 and KLB protein expression were determined by immunoblot assays. For (C) and (D), the respective relative protein levels were calculated relative to DMSO control after normalizing the protein band density to that of the loading control.

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Molecular Cancer

ISSN: 1476-4598