Figure 6
From: Loss of HLTF function promotes intestinal carcinogenesis
Down-regulation of HLTF in human HCT116 colon cancer cells promotes tumor growth. (A) Western blot analysis with anti-HLTF antibody on the cell lysates prepared from HLTF methylated human colon cancer cells (RKO and SW480) and HLTF unmethylated human colon cancer cells (HCT116). (B) Western blot analysis on the cell lysates prepared from HCT116 cells that were stably integrated with scramble control shRNA and HLTF shRNAs. HLTF expression was efficiently inhibited by two independent HLTF shRNAs. (C) Representative image of subcutaneous tumors formed from HCT116Scramble, HCT116HLTFshRNA1 and HCT116HLTFshRNA2 cells in Rag1-/-/IL2-/- immunodeficient mice. (D) Haematoxylin-eosin staining demonstrates that both HLTF knockdown tumors displayed tumor cell invasion into the muscle layer (indicated by arrows) which was not typically observed in the scramble control tumors. HLTF knockdown tumors also showed low expression of HLTF as detected by immuno-staining with anti-HLTF antibody.