(A-D) A-1, QRT-PCR, SDC-2 mRNA levels in nerve invasive cancer cells; A-2, SDC-2 protein levels in cell lysates (c/l); GAPDH as a loading control; "Fold expression" has been calculated using densitometry of the immunoblots which were normalized to GAPDH. B-1, QRT-PCR, SDC-2 mRNA levels in PDAC cell lines (normal colon as a positive control). B-2, SDC-2 protein levels in PDAC cell lysates (normal colon as positive control). C, QRT-PCR, SDC-2 mRNA levels in human normal pancreas (donor), CP and PDAC tissues. D, enzymatically treated SU8686 cell lysates; NT = not treated; (1) = 6 M urea in sodium acetate ph 4,5 treated; (2) = 6 M urea in sodium acetate ph 4,5 plus 1 mU/ml Heparinase III; (3) = 1 mU/ml Heparinase III treatment; and (3) = 2 mM/ml Heparinase III treatment.