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Figure 4 | Molecular Cancer

Figure 4

From: Osteoblast-secreted collagen upregulates paracrine Sonic hedgehog signaling by prostate cancer cells and enhances osteoblast differentiation

Figure 4

AA increases ALP activity in MC3T3 pre-osteoblasts. (A) MC3T3 cells were grown in 6-well plates for 7 or 14 days with or without exogenous AA. ALP activity was determined in cell lysates at indicated time points. ALP activity in the presence of AA (filled bars) was compared to that without AA (open bars) within each time point. Data are means ± SD of 4 replicates from 2 independent samples per group. *, P < 0.05. (B) MC3T3 cells were grown in 6-well plates for 14 days with or without AA and the collagen synthesis inhibitor 3,4-dehydro-L-proline (DHP; 0.5 mM). Cultures were then stained for ALP activity. Results are representative of 2 independent experiments. (C) MC3T3 cells were grown in 96-well plates for 24 h in the presence of varying concentrations of DHP. Absorbance measurements are in direct proportion to the number of living cells. Data are means ± SD of 4 replicate wells per treatment.

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Molecular Cancer

ISSN: 1476-4598