Figure 4
From: Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion
(A) Galectin-1 transfection does not alter U87MG attachment to fibronectin. Attachment to fibronectin-coated 96-well plates was quantitated with an MTT assay with and without a media change in the middle of the assay. Data were normalized to those of the parental line. One representative assay is presented. (B) Galectin-1 transfected clones do not proliferate faster than controls. The change in MTS signal from day 1 is plotted against time. (C) Over-expression of galectin-1 promotes an increased rate of two-dimensional migration. Galectin-1 transfected clones were compared to their GFP control counterparts. (Westerns controlled for loading by β-actin IB). (D) Over-expression of galectin-1 promotes invasion. All cell counts were normalized to the parental cell line data. (Westerns controlled for loading by β-actin IB)