Over-expression of galectin-1 in orthotopic xenografts decreases host survival. Orthotopic xenografts of parental U87G and galectin-1 transfected cells were established in nude mice. Ten mice were assigned to each cell line, and each mouse received intracranial injection of 1 x 106 cells under general anesthesia. At the onset of neurological symptoms, animals were sacrificed in accordance with the Mayo Clinic IACUC. Survival curves were generated from those animals (38 of 40) developing tumors (7 of 20 acGFP-only).