Figure 3

PHA-739358 eliminates activity of both Bcr/Abl and Aurora kinase. (A). BLQ1 (Ph-positive T315I mutation) and UCSF02 (Ph-positive no mutation) cells were incubated with the indicated concentrations of PHA-739358 with or without dasatinib (100 nM) for 24 hours. Phosphorylation status of Bcr/Abl, Stat5, Crkl and Src was assessed by Western blot. Blots were stripped and reprobed with Bcr, Src and Gapdh as loading controls. (B). BLQ1 and US6 cells were exposed to 1 μM PHA-739358 for 24 and 48 hours and the percentage of phospho-histone H3 (Ser10) positive cells was defined by flow cytometry.