Figure 5

(A) Effects of cisplatin and/or UV-C on the localization of EGFR in SW480 cells. SW480 cells were first labeled for 15 min at 37°C with anti-EGFR antibodies. They were then exposed to 10 J/m² of UV-C (panels 6–10 and 11–15, respectively) or not (panels 1–5, respectively), followed by the treatment with (panels 1–5 and 11–15, respectively) or without (panels 6–10, respectively) 10 μM of cisplatin for the indicated periods at 37°C. After fixation and permeabilization, the cells were stained with Alexa 488® conjugated anti-mouse secondary antibody for EGFR (green signal) and DAPI (blue signal) for 1 h, and then examined by fluorescence microscope. (B) Effects of cisplatin and/or UV-C on the amount of cell surface EGFR in SW480 cells. SW480 cells were first labeled for 15 min at 37°C with an anti-EGFR antibody that recognizes the extracellular domain of the EGFR. They were then exposed to 10 J/m² UV-C (lanes 3 and 4) or not (lanes 1 and 2), followed by the treatment with (lanes 2 and 4) or without 10 μM cisplatin (lanes 1 and 3) for the indicated periods at 37°C. The amount of cell surface EGFR was then measured by ELISA. The asterisks (* and **) indicate significant decrease (p < 0.05) with respect to the control (lane 1, respectively). For additional details see Materials and methods.