Figure 6

Reduction of AKT phosphorylation by rolipram in HCT116 cells grown in 3 DC. (A) Western blotting of AKT phosphorylation (p-AKT) at Ser473 and Thr308, and pan-AKT for HCT116 cells treated with DMSO alone or rolipram at day 6 in 2-D or 3-D culture. (B) The quantitative analyses of the level of p-AKT (Ser473) in HCT116 cells treated with DMSO alone or rolipram at day 6 in 3 DC. White bar represents the relative intensity of the signal for HCT116 cells treated with rolipram, normalized by the signal for HCT116 cells treated with DMSO alone (black bar). *, P < 0.01. (C) Upper panel shows western blotting of p-AKT (Ser473) and pan-AKT for HCT116 cells stably transfected with control shRNA or PDE4B2-shRNAs at day 6 in 2-D or 3-D culture. Lower panel shows the quantitative analyses of the level of p-AKT in HCT116 cells stably transfected with control shRNA or PDE4B2-shRNAs at day 6 in 3 DC. White bar represents the relative intensity of the signal for HCT116 cells stably transfected with PDE4B2-shRNAs, normalized by the signal for HCT116 cells stably transfected with control shRNA (black bar). *P < 0.05.