Figure 2
Inactivation of Flna reduces tumor burden in a Kras2 G12D-induced lung cancer in mice. (A) Photographs of lungs 12 weeks after inhalation of adenoviral vector encoding Cre (Ad-Cre). Note the extensive tumor lesions (white areas) on the surface of lungs from Flnao/+Kras2G12D/+ male mice and the relatively normal appearance of the lungs from Flnao/flKras2G12D/+ male mice. (B) Hematoxylin & Eosin (H&E)-stained sections of representative lesions in lungs from Flnao/+Kras2G12D/+ and Flnao/flKras2G12D/+ male mice. Original magnifications × 20. (C) Computer-assisted image analysis of H&E-stained lung tumor areas (upper panels) using artificial colors (lower panels). Yellow color represents lung tumor areas, whereas blue color indicates normal pulmonary structures and airways. Original magnifications × 5. (D) Mean ± SD values of percentage of lung tumor areas from entire lower right and left lung lobes sectioned at five different levels. Each group consisted of at least 5 mice. Student’s t test, *P < 0.05. (E) Immunohistochemical staining of Flnao/+Kras2G12D/+ and Flnao/flKras2G12D/+ lung tumor areas using an anti-FLNA antibody. Red color represents immunohistochemical FLNA-positivity, whereas blue color indicates nuclear counterstaining. Original magnifications × 20 and × 100 (insets). (F) Immunofluorescence detecting of endothelial cells in lung tumors by an antibody against PECAM (red). Nuclear DAPI staining (blue). Original magnifications × 20.