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Figure 2 | Molecular Cancer

Figure 2

From: Interferon regulatory factor 4 binding protein is a novel p53 target gene and suppresses cisplatin-induced apoptosis of breast cancer cells

Figure 2

p53 attenuates IBP expression. (A and C) The expression of IBP and p53 in MCF-7 cells were tested by Western blot after Ad-p53 infection at different time points (A) or p53 RNAi lentiviruses infection (C). Ad-GFP or p53 RNAi cont. was a respective control. (B and D) The expression of IBP was detected by Western blot in MCF-7 cells treated with different concentrations of Nutlin-3 (B) or pifithrin-α (D). GAPDH and p21 served as controls. (E) Real-time PCR analysis of the expression of IBP in MCF-7 cells treated with Ad-p53 (96 h), p53-RNAi, Nutlin-3(10 μmol/L for 8 h ) or pifithrin-α (30 μmol/L for 24 h); GAPDH was used as a control. * , p < 0.01.

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Molecular Cancer

ISSN: 1476-4598