Figure 2

Activated Akt inhibits TGFβ-induced EMT in an isoform-redundant manner. MCF10A cells were infected with retroviruses to express empty vector (V), Akt1 (1), Akt2 (2) or Akt3 (3) and then cultured in the absence or presence of TGFβ (2 ηg/ml, R & D research) for 3 days followed by RT-qPCR to analyze EMT-associated transcripts. Significant changes in the experimental groups compared to vector control were marked with * or with # (respectively denoting without or with TGFβ treatment) if p < 0.05.