Figure 4

Rab31 overexpression reduces the adhesive capacity of human breast cancer cells in vitro. Stably transfected MDA-MB-231 cells (A) and CAMA-1 cells (B) were seeded on collagen type IV-coated microtiter plates. After 2 h of cell cultivation, the number of adherent cells was monitored by the hexosaminidase activity assay. At least 5 independent experiments were performed in triplicates each. The results are given in % relative to the cell number of adherent vector-transfected control cells. Whisker box plots indicate the 25th and 75th percentile, the vertical bars indicate the 10th and 90th percentile. The median value of at least 5 experiments is indicated by a bar within the box. Statistically significant differences (p < 0.05) to the vector control group are indicated by an asterisk, differences between other groups are indicated by a line with an asterisk.