Analysis of MUC1 and c-Met expression in normal and cirrhotic liver tissue, and HCC. Negative MUC1 expression in normal hepatocytes and positive MUC1 expression was localized to bile ducts (×400) (A). Cirrhotic liver tissue showed weak, diffuse cytoplasmic MUC1 staining (×400) (B). HCC displayed intense MUC1 staining (×400) (C). Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for MUC1 (D). c-Met expression in normal liver tissue showed very weak or no immunoreactivity (×400) (E). Cytoplasmic c-Met staining in hepatocytes in the cirrhotic liver tissue (×400) (F). HCC displayed positive cytoplasmic and strong membranous c-Met staining (×400) (G). Comparison of the ratios of positive staining for c-Met in normal liver, cirrhotic liver, and HCC tissues (H).