Figure 3

Identification of miR-146a targets. (A) Predicted miR-146a target transcripts are significantly down-regulated after miR-146a transfection compared to expressed genes that are not predicted targets of miR-146a (P < 4.6e-11, two-tailed Wilcoxon rank-sum test). (B) Top 10 words most correlated with down-regulation (correlation Z-scores are indicated to the left of each word, all 10 words have an estimated FDR < 1e-6). Capital letters highlight the miRNA seed region, Watson-Crick base-pairs (blue), mismatches (red). The word strongest correlated with down-regulation was the seed site complementary to mature miRNA bases 2-7/8. (C) Transcripts, having 6mer or 7mer miR-146a seed sites in the 3’UTR, that are most down-regulated upon miR-146a transfection are shown. These are potential direct miR-146a targets. Expression is given in log2 fold change. The 3’UTR 6 m/7 m columns indicate numbers of 6- or 7mer miR-146a seed sites in the 3’UTR.