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Figure 2 | Molecular Cancer

Figure 2

From: EGFR/Src/Akt signaling modulates Sox2 expression and self-renewal of stem-like side-population cells in non-small cell lung cancer

Figure 2

SP cells express stem cell-like markers. (A) Western blot analysis on SP and MP cells from A549, H1650 and H1975 cell lines for the indicated proteins. β-Actin was used as the loading control. (B, C, D) SP and MP cells were analyzed by real time qRT-PCR method for the expression of Twist, Slug and Snail genes. Average (±SD) fold change between MP and SP cells were plotted. (E, F, G) SP and MP cells were analyzed by real time qPCR method for the expression of ABCG2, Oct4, Sox2 and Nanog genes. Average (±SD) fold change between MP and SP cells were plotted. (H) The spheres formed in self-renewal assays of H1650 SP cells were stained for the expression of ABCG2, Oct4, Sox2 and Nanog. Confocal image of one of the z-stacks for a sphere is presented. DAPI was used to stain the nucleus.

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