(A) Mcl-1 mRNA measured by reverse transcription PCR in Jeko-1 cells with (1.5 μmol/L) or without (control) P276-00 treatment at indicated time points (B) Densitometric representation of RT-PCR indicating statistically significant differences compare to control (C) Western blot analysis of protein expression at 48 h after transfection with siRNAs (D) Effect of cyclin D1 and Mcl-1 siRNA in Jeko-1 cells. Cells were transfected with siRNAs i.e. control siRNA (control) and cyclin D1 (cyc D1) or Mcl-1 or the combination of both the siRNAs (cyclin D1 + Mcl-1) with P276-00. Columns, mean percentage of live cells after transfection (treated) from two independent experiments; bars, SD. **, *** significantly different from control siRNA transfected group (** p = < 0.01; *** p = < 0.001).