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Figure 4 | Molecular Cancer

Figure 4

From: Epidermal growth factor (EGF) and interleukin (IL)-1β synergistically promote ERK1/2-mediated invasive breast ductal cancer cell migration and invasion

Figure 4

Knockdown of ERK1/2 inhibits the synergistic activation of AP-1 by EGF and IL-1β in IBDC cells. EGF and IL-1β stimulation increased the activity of an AP-1 luciferase reporter gene in BT474 cells; however, EGF plus IL-1β synergistically increased AP-1 luciferase activity. Transfection of BT474 cells with ERK1/2 siRNA decreased EGF or IL-1β, or EGF plus IL-1β-induced AP-1 activation in a dose-dependent manner. ** or ◇◇, P < 0.05 vs. control siRNA and AP-1 luc-transfected cells stimulated with EGF or IL-1β alone; ▼▼ and ΔΔ, P < 0.05 vs. control siRNA and AP-1 luc-transfected cells stimulated with EGF plus IL-1β; relative luciferase activity was normalized against B-gal.

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Molecular Cancer

ISSN: 1476-4598