Figure 4

Elk-1 is activated by ascites and its activation is controlled by ERK1/2. (A) Cells were treated with OVC415 ascites (10%) for up to 240 min and the expression and phosphorylation of Elk-1 was assessed by immunoblot. Activation of Elk-1 was detected with an anti-phospho-Elk-1 antibody specific for Ser383. Tubulin was used as a control for loading. (B) Cells were treated with U0126 for 1 h before the addition of OVC415 ascites (10%). Lysates were obtained 1 h after the addition of ascites and immunoblot analysis was performed with anti-phospho-ERK1/2 and anti-phospho-Elk-1 antibodies. Representative data from three independent experiments.