Figure 6From: Combined targeting of AKT and mTOR synergistically inhibits proliferation of hepatocellular carcinoma cellsAKT isoforms are differentially activated in human HCC tissue samples. (A) Ten human HCC tissue samples obtained from specimen removed during surgery were analyzed by Western blot for expression of mTOR and AKT signaling proteins. HSC70 served as loading control. (B) Same samples, including two corresponding controls from surrounding normal liver tissue, were analyzed by AKT isoform specific in vitro kinase assay as described before. IgG served as loading control.Back to article page