Figure 2
From: IL-1β promotes stemness and invasiveness of colon cancer cells through Zeb1 activation
IL-1β induces sphere formation and proliferation of colon cancer cells in serum-free medium (SFM). (A) Representative images of HCT-116 and HPCC cells in the absence or presence of IL-1β. HCT-116 and HPCC cells (1 cell/μl) were cultured in 96-well plates containing 100 μl SFM in each well with or without IL-1β for seven days. The number of spheres was counted under a microscope and size was measured using ImageJ (B and C). Then sphere cells were dissociated and stained with Trypan blue and counted under a microscope. Error bars represent SEM. *p < 0.05.