Figure 3
From: Highly efficient site-specific transgenesis in cancer cell lines
Fidelity of the docking-incoming system. (A) Position of the primer pairs used for screening of integration (Int F and Int R) as well as the tk probe used for southern analysis. (B) PCR amplification of the integration junction using primers recognizing the att L site and the neoR probe. Sixty-six colonies (60 colonies for Pc-3-A7 and 6 for SKOV-3-13) were screened and all had the correct integration site. Analysis was done using a multicomp agarose gel. (C) Southern blot analysis of subclones derived by integration of two different incoming vectors (IncCAG-transgene; lanes 2–7, and IncTRE-transgene; lanes 9–13) into line Pc-3-A7 (lanes 2–7) and SKOV-3-13 (lanes 9–13). Genomic DNA was digested with BamH I and the tk probe was used. Original Pc-3-A7 and SKOV3-13 DockZ lines were also included in lanes 8 and 14, respectively. 1-Kb marker is shown in the first lane.