Figure 5
From: Highly efficient site-specific transgenesis in cancer cell lines
Generation of an inducible system. (A) Plasmid encoding the second generation of rtTA under the control of CMV. (B) EGFP expression after integration of IncTRE-EGFP into Pc-3-A7 rtTA sublines #23 and #25, before and after induction with dox for 24 h and 48 h. bf; brightfield, gfp; fluorescent. (C) Histogram plots of EGFP expression of three isogenic clones derived after integration of IncTRE-Flt1-Fc-IRES-EGFP into Pc-3-A7-23. (D) Expression levels of (i) Flt1-Fc and (ii) VEGF in the supernatant of Pc-3-A7-23 stably transfected with IncTRE-EGFP or IncTRE-Flt1Fc-IRES-EGFP, before (white columns) and 48h after (grey columns) dox induction (n=3). (E) Tumour volume of Pc-3-A7-23 xenografts stably transfected with IncTRE-EGFP (empty grey squares, n=4) or IncTRE-Flt1Fc-IRES-EGFP (filled blue squares, n=4). Arrow indicates the time point at which the mice were switched from normal chow to doxycycline containing food pellets.