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Table 1 Plasma membrane associated proteins identified from the MDA-MB-231 cell line using LPI™ FlowCells

From: Combining phenotypic and proteomic approaches to identify membrane targets in a ‘triple negative’ breast cancer cell type

Protein name

Reproducibility (n = 5)

Pept. seq.

Seq. cov. (%)

CD151 antigen

2

2

5.5

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12

5

3

34.7

Basigin

5

3

11.9

Annexin A1

5

2

5.5

CD44 antigen

5

3

5.0

Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

5

4

4.8

Integrin a6

5

3

3.2

CD97 antigen

5

2

3.1

Integrin a3

5

2

1.8

Integrin a2

5

2

1.7

Ras-related protein Rab-35

4

3

15.4

Ephrin type-A receptor 2

4

5

5.3

Protein Shroom3

4

1

0.5

Retinoic acid-induced protein 3

3

2

5.6

MUC18

3

2

2.6

Integrin a5

3

1

1.0

Ras-related protein Rap-2b

2

2

12.0

Annexin A5

2

1

2.8

Poliovirus receptor-related protein 2

2

1

1.5

EGF receptor

2

2

1.3

Caveolin-1

5

3

15.2

Myoferlin

5

15

7.7

Myosin-9

4

3

1.5

Myosin-Ic

3

2

2.0

Plectin

5

2

3.4

HLA class I histocompatibility antigen, A-2 alpha chain

5

2

9.0

HLA class I histocompatibility antigen, B-13 alpha chain

5

2

2.5

Pyruvate kinase isozymes M1/M2

5

2

2.1

CD81 antigen

3

2

8.5

Calreticulin

5

2

11.8

Protein disulfide-isomerase

5

2

5.1

Mortalin

2

1

1.3

CD73

5

2

5.2

Monocarboxylate transporter 4

5

2

10.5

Large neutral amino acids transporter small subunit 1

5

2

6.7

Plasma membrane calcium-ransporting ATPase 1

5

2

5.2

High affinity cationic amino acid transporter 1

3

1

1.3

  1. Proteins were identified from the SwissProt database with Mascot. The table summarizes the sequence coverage, the number of peptides sequenced and the number of runs each protein was detected (n = 5).