Skip to main content

Advertisement

Table 1 Plasma membrane associated proteins identified from the MDA-MB-231 cell line using LPI™ FlowCells

From: Combining phenotypic and proteomic approaches to identify membrane targets in a ‘triple negative’ breast cancer cell type

Protein name Reproducibility (n = 5) Pept. seq. Seq. cov. (%)
CD151 antigen 2 2 5.5
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 5 3 34.7
Basigin 5 3 11.9
Annexin A1 5 2 5.5
CD44 antigen 5 3 5.0
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas 5 4 4.8
Integrin a6 5 3 3.2
CD97 antigen 5 2 3.1
Integrin a3 5 2 1.8
Integrin a2 5 2 1.7
Ras-related protein Rab-35 4 3 15.4
Ephrin type-A receptor 2 4 5 5.3
Protein Shroom3 4 1 0.5
Retinoic acid-induced protein 3 3 2 5.6
MUC18 3 2 2.6
Integrin a5 3 1 1.0
Ras-related protein Rap-2b 2 2 12.0
Annexin A5 2 1 2.8
Poliovirus receptor-related protein 2 2 1 1.5
EGF receptor 2 2 1.3
Caveolin-1 5 3 15.2
Myoferlin 5 15 7.7
Myosin-9 4 3 1.5
Myosin-Ic 3 2 2.0
Plectin 5 2 3.4
HLA class I histocompatibility antigen, A-2 alpha chain 5 2 9.0
HLA class I histocompatibility antigen, B-13 alpha chain 5 2 2.5
Pyruvate kinase isozymes M1/M2 5 2 2.1
CD81 antigen 3 2 8.5
Calreticulin 5 2 11.8
Protein disulfide-isomerase 5 2 5.1
Mortalin 2 1 1.3
CD73 5 2 5.2
Monocarboxylate transporter 4 5 2 10.5
Large neutral amino acids transporter small subunit 1 5 2 6.7
Plasma membrane calcium-ransporting ATPase 1 5 2 5.2
High affinity cationic amino acid transporter 1 3 1 1.3
  1. Proteins were identified from the SwissProt database with Mascot. The table summarizes the sequence coverage, the number of peptides sequenced and the number of runs each protein was detected (n = 5).