Direct comparison with normal matched tissue can be performed
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Extremely reliant on quality of membrane preparation and extraction of membrane proteins
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Screening antibodies can assign a potential mechanism of action
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High cell requirement
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Reduced cell requirement compared to hybridoma approach
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Isolation of relatively low affinity antibodies and poor target identification success rate- requirement for complementary techniques for success of target identification
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Sample fractionation possible and total survey of the membranome
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Reliant on database annotations
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Isolation of high affinity antibodies
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Dominance of single targets and antibodies
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Ability to perform initial screen against multiple cell types with relative ease
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No function or mechanism of action associated with antigens identified
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Functional in phenotypic screens
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No ability to deselect against abundant antigens or comparator cell types
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Screening can assign a potential mechanism of action
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High target identification success rate
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Ability to avoid dominance – can deselect against abundant antigens and comparator cell types
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Isolation of antibodies that can be used for target validation or as a therapeutic candidates
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Isolation of antibodies that can be used for target validation
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Potential to identify target and therapeutic candidate
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