Skip to main content

Table 2 Comparison of the advantages and disadvantages of membranome target discovery techniques

From: Combining phenotypic and proteomic approaches to identify membrane targets in a ‘triple negative’ breast cancer cell type

Protein profiling using LPI™ FlowCells and LC-MS/MS

Hybridoma-based phenotypic antibody screening

Phage display-based phenotypic antibody screening







Direct comparison with normal matched tissue can be performed

Extremely reliant on quality of membrane preparation and extraction of membrane proteins

Screening antibodies can assign a potential mechanism of action

High cell requirement

Reduced cell requirement compared to hybridoma approach

Isolation of relatively low affinity antibodies and poor target identification success rate- requirement for complementary techniques for success of target identification

Sample fractionation possible and total survey of the membranome

Reliant on database annotations

Isolation of high affinity antibodies

Dominance of single targets and antibodies

Ability to perform initial screen against multiple cell types with relative ease


No function or mechanism of action associated with antigens identified

Functional in phenotypic screens

No ability to deselect against abundant antigens or comparator cell types

Screening can assign a potential mechanism of action


High target identification success rate


Ability to avoid dominance – can deselect against abundant antigens and comparator cell types


Isolation of antibodies that can be used for target validation or as a therapeutic candidates


Isolation of antibodies that can be used for target validation


Potential to identify target and therapeutic candidate

  1. Comparing LPI™. FlowCells and LC-MS/MS with hybridoma-based phenotypic antibody screening and phage display-based antibody screening.