Figure 4

The expression of N-Cadherin in the presence of integrin inhibitors. (A) Western blot and densitometric analysis of N-Cadherin expression in RWPE1, PC3, HS5 and co-cultured cells (PC3 + HS5) in the presence and absence of integrin inhibitors. Dashed line indicates samples taken from a separate membrane. (B-B’) Immunostaining of N-Cadherin in HS5 and (C) PC3 cells. (C’) Immunostaining of N-Cadherin in PC3 cells in the presence of α6 and β1 inhibitors. (D-D”) Expression of N-Cadherin in co-cultures, in the presence of PC3 cells, N-Cadherin expression was evident on HS5 cells (STRO-1; unfilled arrowheads). (E-E’) In the presence of α6 and β1 inhibitors, HS5 cells (STRO-1; unfilled arrowheads) continued to express N-Cadherin. (F-F”) DIC image of HS5 cells treated with standard media, PC3-treated media or 3T3-treated media. (G) Western Blot and densitometric anaylsis of N-Cadherin expression in HS5 cells using standard, PC3-treated and 3T3-treated media over a 9 day period. Scale bar = 40 μm.