Figure 5
From: Bone-stromal cells up-regulate tumourigenic markers in a tumour-stromal 3D model of prostate cancer

The expression of vimentin in the presence of integrin inhibitors. (A) Western blot and densitometric analysis of vimentin expression in RWPE1, PC3, HS5 and co-cultured cells (PC3 + HS5) in the presence and absence of integrin inhibitors. (B-B’) Immunostaining of vimentin in PC3 cells under (B) control and (B’) β1 inhibitor conditions where vimentin was re-distributed to the membrane (unfilled arrowhead). (C-C’) Expression of vimentin in co-cultures. Vimentin was present in the cytoplasm of HS5 (STRO-1; filled arrowheads) and PC3 (Cell Mask; unfilled arrowheads) cells. (D-E’) In the presence of α6 or β1 inhibitors, HS5 (STRO-1; filled arrowheads) and PC3 (Cell Mask; unfilled arrowheads) cells continued to express vimentin in the cytoplasm. Scale bars = 40 μm.