The expression of vimentin in the presence of integrin inhibitors. (A) Western blot and densitometric analysis of vimentin expression in RWPE1, PC3, HS5 and co-cultured cells (PC3 + HS5) in the presence and absence of integrin inhibitors. (B-B’) Immunostaining of vimentin in PC3 cells under (B) control and (B’) β1 inhibitor conditions where vimentin was re-distributed to the membrane (unfilled arrowhead). (C-C’) Expression of vimentin in co-cultures. Vimentin was present in the cytoplasm of HS5 (STRO-1; filled arrowheads) and PC3 (Cell Mask; unfilled arrowheads) cells. (D-E’) In the presence of α6 or β1 inhibitors, HS5 (STRO-1; filled arrowheads) and PC3 (Cell Mask; unfilled arrowheads) cells continued to express vimentin in the cytoplasm. Scale bars = 40 μm.